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Detecting EMT and stemness-related genes expression by western blot.
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posted on 2014-04-04, 02:54 authored by Yuqin Zhang, Lin Zheng, Jing Huang, Fei Gao, Xiaoshan Lin, Lian He, Dan Li, Zhijun Li, Yi Ding, Longhua ChenWestern blot analyzed the EMT-related genes like E-cadherin, ZO-1, Vimentin and N-caherin and stemness-related genes such as ABCG2, SOX2 and Oct4 in miR-124-tranfected cells and miR-124-PRRX1 co-transfected cells compared with control group. Data suggested over-expression of miR-124 could down-regulate Vimentin, N-cadherin, ABCG2, SOX2 and Oct4 expression and up-regulate E-cadherin, ZO-1 expression, while over-expression of PRRX1 could rescue this effect.
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cell biologyMolecular cell biologygeneticsepigeneticsRNA interferencegene expressionimmunologyClinical immunologyGastroenterology and hepatologyGastrointestinal cancershematologyoncologyCancers and neoplasmscarcinomasadenocarcinomasColon adenocarcinomaGastrointestinal tumorsRectal canceremtstemness-relatedgenes
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