Deregulation of the mitochondrial pathway in the OPMD Drosophila model.

A) Number of deregulated genes in the transcriptome of PABPN1-17ala-expressing muscles using microarrays. B) Venn’s diagram of overlapping down-regulated genes at days 2, 6 and 11. C) GO term enrichment in down-regulated genes in PABPN1-17ala-expressing muscles. D) Number of nuclear genes encoding mitochondrial respiratory chain complex subunits down-regulated in PABPN1-17ala-expressing muscles. E) Quantification of levels of mRNAs encoding mitochondrial respiratory chain subunits of complexes I and II in control and PABPN1-17ala-expressing muscles at day 2, using RT-qPCR. mRNA levels were normalized to sop mRNA. Similar results were obtained when normalization was with Cpr100A mRNA which is expressed in thorax cuticle. Means are from three biological replicates, error bars represent standard deviation. * p-value <0.05, *** p-value <0.001, ns: not significant, using the Student’s t-Test.