Dependency of the established erythroid progenitor cell lines on externally supplied culture factors.

(A) The survival and proliferation of HiDEP-1 cells are dependent on DOX (HPV16-E6/E7) and EPO and partially dependent on DEX. (B) The survival and proliferation of HUDEP-1 cells are dependent on DOX (HPV16-E6/E7) and SCF and partially dependent on EPO. (A, B) DOX, doxycycline; expression of HPV16-E6/E7 is induced by DOX. SCF, stem cell factor. EPO, erythropoietin. DEX, dexamethasone. ALL, cells were cultured in the presence of DOX, SCF, EPO and DEX. –DOX, –SCF, –EPO, –DEX, cells were cultured after deprivation of DOX, SCF, EPO and DEX, respectively. Dependencies of other cell lines on externally supplied culture factors are summarized in Table S1.