Deletion of the <i>S. bayanus HMR-E</i> resulted in loss of silencing.

<p>Mating test of <i>MAT</i>α strains to <i>MAT</i>a tester strain (JRY2726). Disruption of silencing changed the mating type of the <i>MAT</i>a strains to nonmating phenotype of a/α diploid. Two independently constructed <i>S. bayanus hmr-e</i> deletion strains (JRY8785, JRY8786) lost silencing to the same extent as the <i>S. bayanus sir2Δ</i> strain. The parental <i>HMR-E</i> strains (JRY8781, JRY8782) mated as efficiently as the <i>S. cerevisiae</i> control (JRY2728).</p>