Deletion of pfkA and pfkB genes in M. tuberculosis.

Schematic representation of the genomic regions of (A) pfkA in WT and ΔpfkA mutant, (C) pfkB in WT and ΔpfkB mutant, and location of restriction sites and probes. (B) and (D) are Southern blots confirming the knockout of pfkA and pfkB genes respectively. res: sites of resolvase; hyg: hygromycin resistance cassette.