Deletion of <i>pfkA</i> and <i>pfkB</i> genes in <i>M. tuberculosis</i>.

<p>Schematic representation of the genomic regions of (A) <i>pfkA</i> in WT and <b><i>Δ</i></b><i>pfkA</i> mutant, (C) <i>pfkB</i> in WT and <i>ΔpfkB</i> mutant, and location of restriction sites and probes. (B) and (D) are Southern blots confirming the knockout of <i>pfkA</i> and <i>pfkB</i> genes respectively. <i>res</i>: sites of resolvase; <i>hyg</i>: hygromycin resistance cassette.</p>