Figure_9.tif (5.35 MB)
DSB-2 and SUN-1 S8P persist when CO formation is impaired.
figure
posted on 2013-08-08, 02:43 authored by Simona Rosu, Karl A. Zawadzki, Ericca L. Stamper, Diana E. Libuda, Angela L. Reese, Abby F. Dernburg, Anne M. VilleneuveImmunofluorescence images of gonads of indicated genotypes from the distal pre-meiotic region to end of pachytene, stained with DAPI and antibodies that recognize DSB-2 and SUN-1 S8P. The zone of DSB-2 and SUN-1 S8P-positive nuclei is extended in: (A) the syp-1 mutant, which is defective for SC formation and for formation of interhomolog COs; and (B, C and D) the zhp-3, msh-5 and cosa-1 mutants, respectively, which are proficient for synapsis and DNA repair but are defective in conversion of DSBs to COs. Scale bar, 15 µm.