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Culture supernatants were used in a high performance liquid chromatography (HPLC) analysis to determine ethanol production and glucose consumption.

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posted on 2011-07-21, 01:15 authored by Nora Grahl, Srisombat Puttikamonkul, Jeffrey M. Macdonald, Michael P. Gamcsik, Lisa Y. Ngo, Tobias M. Hohl, Robert A. Cramer

After 48 hours under hypoxic conditions, ethanol was detected along with a decrease in glucose concentration. Ethanol concentrations decrease over time with a peak at 48 hours. Each value represents mean ± standard error (n = 2 independent cultures). *,**,#p<0.05.

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