Cross-contamination estimates from the positive controls in our experimental design.

A. Comparison of the proportion of individuals displaying “Unexpected Reads” (URs) (i.e reads not assigned to P. falciparum, P. malariae or P. ovale) between PCR plates where positive individuals were amplified (PCR plates with positive controls) and those without positive controls (PCR plates without positive controls). B. Comparison of the average number of “Unexpected Reads” (URs) per individual in PCR plates with (PCR plates with positive controls) and without the positive controls (PCR plates without positive controls).