Figure_7.tif (2.8 MB)
Confocal immunofluorescence NF-κB p65 staining in pulmonary vascular cells.
figure
posted on 2013-10-04, 02:41 authored by Laura C. Price, Gaetano Caramori, Frederic Perros, Chao Meng, Natalia Gambaryan, Peter Dorfmuller, David Montani, Paolo Casolari, Jie Zhu, Konstantinos Dimopoulos, Dongmin Shao, Barbara Girerd, Sharon Mumby, Alastair Proudfoot, Mark Griffiths, Alberto Papi, Marc Humbert, Ian M. Adcock, S. John WortConfocal immunofluorescence staining of acetone-fixed frozen lung specimens using NF-κB KAC310 AlexaFluor488 (cyan-green), and DAPI (blue) counterstaining, showed little positive nuclear staining in control lung specimens (A), and marked nuclear staining in idiopathic PAH (B and D). Antibody negative control staining of a control specimen is shown in C. Some green autofluorescence is seen with collagen fibers. Magnification x400 (insert x2400).
