Complementation of the <i>S. pombe Δcoq7</i>, <i>Δcoq5,</i> and <i>Δcoq8</i> strains by Hs<i>COQ7,</i> Hs<i>COQ5</i> and Hs<i>COQ8</i>.

<p>(A) HPLC analyses of lipid extracts from the KH7 (<i>Δcoq7</i>) strain expressing <i>S. pombe coq7</i>, Hs<i>COQ7</i>, or Hs<i>COQ7</i> containing a mitochondrial targeting sequence (Mt-signal). The flow speed was doubled to separate the two peaks clearly. DMQ10, demethoxyubiquinone. (B) HPLC analyses of lipid extracts from the KH5 (<i>Δcoq5</i>) strain expressing <i>S. pombe coq5</i> or Hs<i>COQ5</i>. CoQ10 was mixed with the lipid extracts from the KH5 (<i>Δcoq5</i>) strain expressing Hs<i>COQ5</i>. Asterisks indicate the intermediate like peaks found in a <i>coq5</i> deletion strain. (C) HPLC analyses of lipid extracts from the KH8 (<i>Δcoq8</i>) strain expressing <i>S. pombe coq8</i> or Hs<i>COQ8</i>. (D) Cell growth of the indicated strains over 72 h. W.T., wild type strain.</p>