Figure_2.tif (4.23 MB)
Comparison of the transduction efficiency of FUIGW, UMG-LV5 and UMG-LV6 carrying the ZNF521 cDNA in human hematopoietic cell lines.
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posted on 2014-12-12, 03:03 authored by Emanuela Chiarella, Giovanna Carrà, Stefania Scicchitano, Bruna Codispoti, Tiziana Mega, Michela Lupia, Daniela Pelaggi, Maria G. Marafioti, Annamaria Aloisio, Marco Giordano, Giovanna Nappo, Cristina B. Spoleti, Teresa Grillone, Emilia D. Giovannone, Raffaella Spina, Francesca Bernaudo, Malcolm A. S. Moore, Heather M. Bond, Maria Mesuraca, Giovanni MorroneThe cell lines K562, HL-60, MV4;11, THP-1, Jurkat and DeFew were infected as detailed in materials and methods with FUIGW, UMG-LV5 or UMG-LV6 viruses carrying 3xFLAG-ZNF521 cDNA as a transgene and EGFP cDNA as a reporter gene. As a control, void FUIGW vector without transgene cDNA was used. (A) Flow-cytometric analysis of EGFP expression in cells exposed to the relevant vectors. The percentages of EGFP-positive cells are indicated. (B) Nuclear and cytosolic extracts were prepared as described in materials and methods and analyzed by Western blotting for FLAG-ZNF521 and EGFP expression respectively. HDAC1 was used as a control for the amounts of extract loaded.
