Comparison of sperm function parameters in mice fed either CD or HFD.

(A) Sperm motility in mice fed either CD or HFD analyzed by CASA. (B) Sperm progressive motility in mice fed either CD or HFD analyzed by CASA. (C) Sperm concentration from cauda epididymis in mice fed either CD or HFD analyzed by CASA. (D) Sperm morphology analysis of CD-fed mice (D1) and HFD-fed mice (D2) using Diff-Quick method. Arrows indicate abnormal spermatozoa. Scale bar = 50 μm. (D3) Normal and abnormal spermatozoa in different forms, Scale bar = 10 μm. (D4) Comparison of sperm abnormality in mice fed either HFD or CD. Data are expressed as mean ± SEM. *P<0.05, **P<0.01.