Comparative infection, replication kinetics, and dissemination of Malaysian CHIKV strains in either Ae. aegypti or Ae. albopictus.

The CHIKV isolates MY/06/37348 (Asian, • and black bars) and MY/08/065 (ECSA, ○ and white bars) were used. Using Ae. aegypti, infection rates of midguts (A), replication in midguts as measured by a TCID50 titration assay with plotted means ± SD of triplicates (B), and dissemination rates in salivary glands (C) were determined. Following infection of Ae. albopictus, midgut infection (D), midgut replication (E), and salivary gland dissemination (F) were measured. Asterisks indicate significant differences (*p<0.05, **p<0.01). The denominator used to calculate midgut infection rates was the number of mosquitoes sampled, and the denominator for dissemination rates was the number of midguts with detectable virus titre. Denominators are shown (n).