Comparative densitometric analysis of pH and calcium-dependent Yops secretion in Y. pseudotuberculosis.

To compare and quantify the Yop secretion efficiency in wild-type Y. pseudotuberculosis under different conditions, Coomassie stained Yop secretion profiles were subjected to densitometric analysis with Multi Gauge software (Fuji Film). The protein YopJ (boxed in Figure 4 and Figure 5E) was selected for quantitative analysis. Growth kinetics in media with different pH’s (Figure S4B) showed attenuation of bacterial growth directly linked to the observed Yop secretion efficiency.

a

secretion efficiency was set to 100%;

b

secretion efficiency at pH 7.5 was set to 100%.