Fig_5.tif (4.02 MB)

Combined PDGFR and HDAC inhibition strikingly reduces proliferation and in vitro invasion of Chordoma cells.

figure

posted on 2015-08-06, 03:18 authored by Dae-Hee Lee, Ying Zhang, Amin B. Kassam, Myung-Jin Park, Paul GardnerPaul Gardner, Daniel Prevedello, Stephanie Henry, Craig Horbinski, Jan H. Beumer, Hussein Tawbi, Brian J. Williams, Mark E. Shaffrey, Merrill J. Egorin, Roger Abounader, Deric M. Park

(A) Cell proliferation assay assessed by MTS method using C18 (PTEN +/-) cells demonstrating significantly decreased proliferation in response to HDAC inhibition and combined treatment with a PDGFR inhibitor compared to controls. This is representative of 3 independent of experiments. *P<0.05, Two sample independent student’s t test. (B) Cell proliferation assays assessed by MTS method using UCH1 (PTEN +/-) and UCH2 (PTEN +/-) cells demonstrating significantly decreased proliferation in response to HDAC inhibition and combined treatment with a PDGFR inhibitor compared to controls. This is representative of 3 independent of experiments. *P<0.05, Two sample independent student’s t test. (C) Representative matrigel invasion assays with the C18 cell line (PTEN +/-) and UCH1 cell line (PTEN +/-), respectively, demonstrating significant decrease in invasion in response to PDGFR inhibition, HDAC inhibition and PDGFR and HDAC inhibition together. These images are representative of 3 independent experiments. *P<0.05, Two sample independent student’s t test compared to untreated control. **P<0.05, Two sample independent student’s t test compared to PDGFR inhibitor alone.