Combinatorial effects of corosolic acid and sorafenib on Huh7 cells.
(A) Dose dependent migration inhibitory effects of corosolic acid and sorafenib on Huh7 cells. The control group was treated with 0.1% DMSO and the experimental groups were treated with indicated compounds. (n = 3, **P < 0.01, ***P < 0.001 compared with the DMSO treated group) (B) Transwell assay were performed to determine anti-migration effect of corosolic acid and sorafenib. The combination index (CI) values were examined at different levels of migration inhibition effect (fa), and the effective combination treatments between corosolic acid and sorafenib (CI < 1) were displayed. (C) Huh7 cells were treated with 0.1% DMSO (control), corosolic acid, sorafenib, or combination of corosolic acid and sorafenib for 30 min, and the lysates were analyzed by western blot. (D) For the in vivo combinatorial study, Huh7 cells (5 × 106) were subcutaneously injected into each mouse. After 7 days, when the tumors reached 50 mm3, the mice were randomized into different groups. CA (2.5, 5 mg/kg), sorafenib (10, 20 mg/kg), or a combination of the two was administrated daily via intraperitoneal injection for 20 days the tumor volume was recorded every 3 to 4 days. (E) Weight of tumor mass (n = 5, **P < 0.01, ***P < 0.001 compared with the DMSO treated control group) and synergistic effects (CI < 1) between different combination group.
