Colocalization among Parkin, Tom20-indicated mitochondria and RFP-LC3-labelled autophagosomes at different times under mitophagy stress.

HeLa cells stably expressing RFP-LC3 transiently expressed GFP-Parkin treated with 10 µM CCCP for different times were fixed and stained with antibodies against Tom20 (blue). Mitochondrial aggregates associating with Parkin signals were shown as cyan in the Merge panels. Bar = 5 µm.