Figure S1 - Coincident Pre-Diabetes Is Associated with Dysregulated Cytokine Responses in Pulmonary Tuberculosis

Gating strategy for estimating frequencies of CD4⁺ and CD8⁺ naïve, central memory and effector memory T cells and natural regulatory T cells. (A) A representative flow cytometry plot showing the gating strategy for estimation of naïve, central memory and effector memory cells from CD4⁺ and CD8⁺ T cells. Naïve cells were classified as CD45RA⁺ CCR7⁺; effector memory cells as CD45RA- CCR7-; and central memory cells as CD45RA- CCR7⁺. (B) A representative flow cytometry plot showing the gating strategy for estimation of nTregs from CD4⁺ T cells. Natural T regulatory T cells (nTregs) were classified as CD4⁺, CD25⁺, Foxp3⁺, CD127dim.

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