Co-localization of EhVps32 with EhADH and Gal/GalNac lectin during pinocytosis.

(A) Confocal microscopy of trophozoites incubated with FITC-dextran for different times and then, treated with mouse αrEhVps32 and rabbit αEhADH antibodies, followed by α-mouse TRITC-labeled and α-rabbit Cy5-labeled secondary antibodies, respectively. (B) Trophozoites incubated with rabbit αrEhVps32 and mouse αGal/GalNac lectin antibodies, and then with α-rabbit TRITC-labeled and α-mouse Pacific blue-labeled antibodies, respectively. (C) Trophozoites incubated with mouse αrEhVps32 antibodies and rhodamine-phalloidin, followed by incubation with α-mouse Pacific blue-labeled secondary antibodies. Only for this last experiment, blue and red channels were digitally inverted.