Fig_5.tif (4.98 MB)
Co-localisation of mAb 3G1 and 2G4 staining with flaviviral replication complexes.
figure
posted on 2015-03-23, 05:40 authored by Caitlin A. O’Brien, Jody Hobson-Peters, Alice Wei Yee Yam, Agathe M. G. Colmant, Breeanna J. McLean, Natalie A. Prow, Daniel Watterson, Sonja Hall-Mendelin, David Warrilow, Mah-Lee Ng, Alexander A. Khromykh, Roy A. HallDual was staining performed on Vero cells infected with WNVKUNV MOI: 10 or mock-infected and acetone-fixed at 48 hours post-infection. Merged images showing co-localisation of A) 3G1 (green), and B) 2G4 (green) with flavivirus NS1 labelled with anti-NS1 mAb (4G4, red), flavivirus NS5 labelled with anti-NS5 mAb (5H1, red) and flavivirus NS3 labelled with polyclonal anti-NS3 rabbit sera (red). Nuclei stained with Hoechst nuclear stain (blue). Images were taken at 100x magnification. Scale bar denotes 10 μm.
