Figure_6.tif (1022.49 kB)
Co-immunoprecipitation of SERCA isoforms with sarcolipin and phospholamban.
figure
posted on 2013-12-16, 03:14 authored by Val A. Fajardo, Eric Bombardier, Chris Vigna, Tahira Devji, Darin Bloemberg, Daniel Gamu, Anthony O. Gramolini, Joe Quadrilatero, A. Russell Tupling(A) SLN and PLN interactions with SERCA1a. (B) SLN and PLN interactions with SERCA2a. (C) SLN and PLN interactions with each other. IP Ab, immunoprecipitating antibody; IP, immunoprecipitate; wash, last wash before elution of immunoprecipitate; UB, unbound represents an aliquot (20 μl from 200 μl total volume) of the sample that did not immunoprecipitate using the IP Ab. Experiments were performed on human vastus lateralis muscle homogenates from 3 separate individuals.
