Clustering results for YES/NO (cAMP), YES/YES (cAMP-Ca2+) and NO/YES (Ca2+) groups.

Ligands group into two big sub-regions: YES/NO and YES/YES and one small sub-region represented by MI3a (NO/YES) ligand. The M3A ligand mainly induced by Ca2+ is separated and does not form a cluster downstream. This representation, which we call ‘PCA correlation ball’, shows both the groups of ligands and their correlations, and also the regulations of gene clusters from the projections of nearby ligands. Bow-graphs in the upper corners show the percent of genes being up- or down- regulated after the expiration of one/both molecules activation limited to first time marks (30 min in B-Cell), and the differences are obvious. Genes functions (‘Function’ or ‘Cellular process’ (GO terms), if ‘Function’ is absent) calculated basing on the enrichment in clusters with p-value<0.05 are shown in the pie-graphs.