Cluster Analysis of the Top 750 Cell Cycle–Regulated Genes

Gene expression data from all experiments were clustered by a hierarchical method (Eisen et al. [10]). Every row represents a gene; every column represents an array. Red signifies up-regulation (i.e., an experiment/control ratio greater than one); green signifies down-regulation (i.e., an experiment/control ratio less than one). Black is a ratio close to one, and grey is missing data. Dynamic range is 16-fold from reddest red to greenest green. The time in hours since the beginning of time-course experiments is shown in black numerals at the top of the Figure. Peaks in septation index are marked with purple rectangles at the top and bottom of the Figure. Clusters discussed in the text are marked with blocks of color. Data for the cdc10-C4 (asynchronous cells with the hyperactive allele cdc10-C4), ace2 OE (asynchronous cells over-expressing ace2), ace2Δ (asynchronous ace2Δ cells), sep1 OE (asynchronous cells over-expressing sep1), and sep1Δ (asynchronous sep1Δ cells) are taken from Rustici et al. [7]. cdc10 encodes a component of the MBF transcription factor; ace2 encodes the Ace2 transcription factor, and sep1 encodes a forkhead transcription factor. Other experiments are described in Materials and Methods.