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Chromodomains of MRG1 and MRG2 specifically bind to tri-methylated H3K4 and H3K36 in vitro.
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posted on 2014-09-11, 02:54 authored by Zhongyuan Bu, Yu Yu, Zepeng Li, Yanchao Liu, Wen Jiang, Ying Huang, Ai-Wu DongA. Binding assays of N-terminal His-tagged chromodomains of MRG1 (His-MRG1N) and MRG2 (His-MRG2N) with H3 peptides containing varying degrees of methylation at K4, K9, K27, or K36. B. ITC measurements of binding between the MRG2 chromodomain and histone peptides. Top panel, MRG2 and H3K4me3 peptide; bottom panel, MRG2 and H3K36me3 peptide.
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Arabidopsis Morf Related Genemodel plant Arabidopsis thalianabind H 3K peptidesMRG 2 proteinstranscription factor CONSTANSimpairs CO bindingH 3K readersH 3K methylationsMRG 2 associatesMRG 2 functioncrystal structure analysesH 3K activitygroup proteins MRG 1histone modification marksMRG 2 actH 3K peptidesMRG 1MRG 2mrg 1 mrg 2FT expressionH 3K ViceFT promoterflorigen FLOWERING LOCUS T
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