Chloroform methanol precipitation of proteins improves cardiac troponin I (cTnI) detection in Western blots.

Cardiac left ventricular tissue from adult rats was homogenized as described in Methods. Homogenate protein (10–50 µg) was (left side) or was not (right side) subjected to chloroform/methanol precipitation prior to its resolution on SDSPAGE. Western blot analyses with anti-cardiac troponin I (cTnI) antisera were then conducted. A typical autoradiograph is shown (top). Histograms represent Mean±S.E. densitometry values, expressed as the percentage of maximal densitometry, from 4 independent experiments. Asterisk shown in the figure indicates statistically significant difference (p<0.01) between 10 µg protein groups for chloroform/methanol precipitated (left) vs. not chloroform/methanol precipitated (right) proteins.