Chemotactic activities for CD45RAhi Treg and CD45ROhi Treg subsets.
Chemotactic indices (CI) of Treg from a healthy donor towards CSF supernatants of 11 MS patients as determined by transwell migration assays. Original input PBMC and migrated cells were stained for CD4, CD25, FOXP3, CD95, CD45RO, and CD45RA, and CIs were calculated by dividing percentages of Treg subsets in CD4+ T-cells of migrated cells by percentages of Treg in CD4+ T-cells in original input PBMCs. Symbols indicate CIs of individual patients. Means are indicated, ** indicates p<0.001.