Characterizations of action potentials in NRVF infected with Ad-Kir2.1, Ad-Na_V1.5 and Ad-Cx43.

A. Action potentials were recorded from triple infected (K/Na/Cx43, 5/20/10 MOI) NRVF (left) but not in K/Cx43 (5/10 MOI) infected NRVF (right). B. Resting membrane potentials in uninfected, K/Na/Cx43 (5/20/10 MOI) infected and K/Cx43 (5/10 MOI) infected NRVF. C. APD₈₀, APD₅₀ and APD₃₀ measured at different pacing cycle length. D. Representative action potential traces shows that resting membrane potentials and APDs are sensitive to BaCl₂ (1 mM) but not 4-AP (1 mM). E. Resting membrane potentials measured before and after BaCl₂ (1 mM) perfusion, and after washout in K/Na/Cx43 NRVF. F. Quantification of APD₃₀, APD₅₀ and APD₈₀ before and after 4-AP perfusion. G. Quantification of APD₃₀, APD₅₀ and APD₈₀ before and after BaCl₂ perfusion. *: p<0.05.