Characterization of the Det1 and YOR283w phosphatase activities.

Optimum pH (A) metal dependence (B) and substrate specificity (C) of Det1 and YOR283w were measured by the release of inorganic phosphate as described in Experimental Procedures. σ, sodium acetate/acetic acid buffer; ⧫, Tris-acetate or Tris-HCl buffer; ν, CAPS/NaOH buffer.