Characterization of Slc39a8(neo/neo) phenotype.

(A) Body weight of embryos and fetuses of the three genotypes–from GD13.5 through GD18.5. N = 15–20 per genotype. *P<0.001, compared with values in Slc39a8(+/+) and Slc39a8(+/neo). In this and subsequent figures, bars and brackets denote means ± S.E.M. Brackets denoting S.E.M. are present in Fig. 1A but are mostly covered up by the size of the symbols. (B) Photographs of (genotyped) Slc39a8(+/+) and Slc39a8(neo/neo) pups at GD11.5 (upper left panel), GD13.5 (upper right), GD16.5 (middle both left & right), and at PND1 (bottom panel). The GD16.5 fetuses (middle left) represent a single litter of 13 pups; the two asterisks in middle left panel denote Slc39a8(neo/neo) homozygotes; the other 11 were Slc39a8(+/+) wild-type and Slc39a8(+/neo) heterozygotes.