ChABC and GF treatments enhance the proliferation and differentiation of oligodendrocyte precursor cells (OPCs) after SCI.

<p>(A–D) Representative confocal images showing triple immunostaining for NG2, Olig2 and BrdU in injured spinal cord. To accurately identify OPCs among BrdU+ cells, we used double labelling for Olig2 and NG2 (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037589#pone-0037589-t002" target="_blank">Table 2</a>). (E–F) Our quantification for BrdU+/Olig2+/NG2+ (arrows in D) revealed a significant increase in the number of OPCs after ChABC+GF treatments relative to vehicle (approximately a three-fold increase in 3 mm rostral point and a four-fold increase in 3 mm caudal point). (G–J) We also used BrdU/Olig2/APC immunostaining combination to mark and quantify mature oligodendrocytes after ChABC and GF treatments. Our analysis showed a positive trend for an increase in the number of newly generated mature oligodendrocytes under ChABC and/or GF treatments compared to vehicle at both 3 mm rostral and caudal points to the injury center (K–L). However, this increased level did not reach a statistically significant level. *p<0.05, n = 6/group.</p>