Cell proliferation assay of purified hGCSF using the M-NFS-60 cell line.

Light microscopy images of M-NFS-60 cells incubated without (A) or with (B–D) 1 ng/mL hGCSF for 48 h. Commercially available hGCSF (B), hGCSF purified from MBP-hGCSF (C), and GCSF purified from PDIb'a'-hGCSF (D) were used. The scale bar represents 100 µm. E. Dose-response curve of M-NFS-60 cells following exposure to different concentrations of purified hGCSF and commercial hGCSF. The number of cells was measured as the OD₅₇₀. •, commercially available hGCSF; ▵, hGCSF purified from MBP-hGCSF; hGCSF purified from PDIb'a'-hGCSF. Data are represented as the mean ± SE of n≥3 of 2 independent experiments. Statistical significance compared to no hGCSF treatment group: *, p<0.05; **, p<0.01; ***, p<0.001.