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Cell death detection in CdSe-exposed and SFN pre-treated hepatocytes.
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posted on 2015-09-24, 03:07 authored by Wei Wang, Yan He, Guodong Yu, Baolong Li, Darren W. Sexton, Thomas Wileman, Alexandra A. Roberts, Chris J. Hamilton, Ruoxi Liu, Yimin Chao, Yujuan Shan, Yongping Bao(A) Pre-treatment with 5 μM SFN abrogated the protective effect with an observable increase the viable cell percentage (double negative, Q1) relative to the non-pre-treated cells. Samples were run on a BD Accuri C6 flow cytometer (Ex 488nm; Em 533/30nm BP & 670nm LP). Data is representative of at least 3 experiments. (B) Effect of SFN on CdSe QD-induced apoptosis and necrosis. Apoptosis and necrosis were determined by flow cytometry using Annexin V/PI staining. *P<0.05, **P<0.01.
