Cell cycle analysis upon transfection of mimic-miR-720 or anti-miR-720.

A–B) Imaging and quantitative analysis of immunocytochemistry for ki-67, showing that miR-720 reduced proliferation of DPCs. Results are representative of 3 independent experiments. ** P<0.01, unpaired t-test, compared to si-control group. C) Knockdown of miR-720 increased the number of ki-67^- quiescent cells. ** P<0.01, unpaired t-test, compared to si-control group. Results are representative of at least 3 independent experiments. D) Co-transfection of anti-miR-720 and DNMT3A or DNMT3B expression constructs showed that either DNMT3A or DNMT3B nullified the decreased proliferation of DPCs induced by miR-720 blockade. Results are representative of at least 2 independent experiments. * P<0.05, ** P<0.01, one-way ANOVA/Tukey.