Figure_5.tif (604.33 kB)
Calpain removed the termini of GFAP to generate the 38-BDP.
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posted on 2014-03-25, 04:28 authored by Zhiqun Zhang, J. Susie Zoltewicz, Stefania Mondello, Kimberly J. Newsom, Zhihui Yang, Boxuan Yang, Firas Kobeissy, Joy Guingab, Olena Glushakova, Steven Robicsek, Shelley Heaton, Andras Buki, Julia Hannay, Mark S. Gold, Richard Rubenstein, Xi-chun May Lu, Jitendra R. Dave, Kara Schmid, Frank Tortella, Claudia S. Robertson, Kevin K. W. WangA. Human GFAP was undigested or digested with calpain (Capn), resolved on SDS-PAGE, stained with Coomassie blue R-250, and the indicated bands were excised. An asterisk marks the 38 kDa GFAP band. B. The N-terminal sequence of each band is shown in the table. C. Calpain cleavage sites (scissors) near the N- and C-termini of GFAP are shown in a schematic drawing of the GFAP. Subdomains (head, rod, tail) and identified/putative phosphorylation sites (P) are indicated, and the predicted 38 kDa calpain fragment is shown. D. Three control and four post-TBI human serum samples (Day 4–10) were probed against human brain lysate (top panel) and cell lysate from HEK cells overexpressing the 38 kDa GFAP-BDP (lower panel).
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BiochemistryproteinsImmune system proteinsantibodiesimmunologyautoimmunityClinical immunologyImmune responseneuroscienceorganismsanimalsvertebratesmammalsrodentsratsDiagnostic medicinehealth carePrimary careneurologyPublic and occupational healthSurgical and invasive medical proceduresModel organismsAnimal modelsremovedterminigfap
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