Figure_4.tif (314.8 kB)

Calpain-associated GFAP breakdown to 38 kDa occurred in vivo.

figure

posted on 2014-03-25, 04:28 authored by Zhiqun Zhang, J. Susie Zoltewicz, Stefania Mondello, Kimberly J. Newsom, Zhihui Yang, Boxuan Yang, Firas Kobeissy, Joy Guingab, Olena Glushakova, Steven Robicsek, Shelley Heaton, Andras Buki, Julia Hannay, Mark S. Gold, Richard Rubenstein, Xi-chun May Lu, Jitendra R. Dave, Kara Schmid, Frank Tortella, Claudia S. Robertson, Kevin K. W. Wang

A. Rats experienced penetrating ballistic-like brain injury (PBBI). Control (naïve, sham) or PBBI brain lysates at 1 day (1D) after injury were immunoblotted and probed with GFAP antibody (Banyan). The 38 kDa GFAP bands were quantified by densitometry. This band was significantly increased compared to controls post PBBI (mean ± SEM; n = 5 per group). Vertical hash marks indicate where intervening lanes were removed. B. Rats were injured by controlled cortical impact (CCI), and injected either with vehicle (n = 3) or the calpain inhibitor SNJ1945 (n = 5). Then brain lysates were immunoblotted and probed with antibodies against αII-spectrin (Enzo) or GFAP (Banyan). Quantification of the SBDP145 and 38 kDa GFAP bands showed that SNJ1945 significantly inhibited formation of SBDP145 and 38 kDa GFAP (mean ± SEM). An asterisk marks the 38 kDa GFAP band throughout.