C-kit⁺ cells after purification.

Panel A showed the representative data of Flow Cytometry (upper row), where c-kit-FITC was shown on x-axis, cell Events# was on y-axis, and the percentages of c-kit⁺ cells was in red histograms. The representative images of ICC from the same patient was displayed in middle row, where c-kit⁺ cells were showed in green (FITC) and nuclei were stained in blue (DAPI) at P2-P10 after c-kit MACS but without Lin-dep. Scale bar = 20 µm. Panel B displayed the Mean±SEM data from ICC (open bar) and Flow Cytometry (filled bar). The percentages of c-kit⁺ cells by ICC and Flow showed no statistical significant difference (ns) compared to P2. n = 3 patients.