Figure_4.tif (652.31 kB)
CRF-induced CREB phosphorylation occurs via a MEK/MAPK-dependent mechanism.
figure
posted on 2011-03-23, 02:43 authored by Christopher M. Stern, Jessie I. Luoma, John Meitzen, Paul G. Mermelstein(A) The CaMK inhibitor KN-93 (2 µM) did not affect CRF-induced CREB phosphorylation (F = 17.65). (B) CRF-induced CREB phosphorylation was blocked by the MEK antagonist U0126 (10 µM; F = 11.97). (C) The L-type Ca2+ channel blocker nifedipine (5 µM) had no effect on CRF-induced CREB phosphorylation (F = 18.50). (D) Chelating intracellular calcium with BAPTA-AM (30 min incubation with 5 µM) failed to block CRF-induced CREB phosphorylation (F = 12.72).
