CRF-induced CREB phosphorylation occurs via a MEK/MAPK-dependent mechanism.

<p>(A) The CaMK inhibitor KN-93 (2 µM) did not affect CRF-induced CREB phosphorylation (<i>F</i> = 17.65). (B) CRF-induced CREB phosphorylation was blocked by the MEK antagonist U0126 (10 µM; <i>F</i> = 11.97). (C) The L-type Ca<sup>2+</sup> channel blocker nifedipine (5 µM) had no effect on CRF-induced CREB phosphorylation (<i>F</i> = 18.50). (D) Chelating intracellular calcium with BAPTA-AM (30 min incubation with 5 µM) failed to block CRF-induced CREB phosphorylation (<i>F</i> = 12.72).</p>

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