CPP-induced autophagy is dependent on FIP200 and Beclin 1.

Wild-type and FIP200−/− MEFs (A–C), and Beclin 1 wild type and Beclin 1-low U251 cells (D–F) stably expressed GFP-LC3. They were treated with EBSS or CPP, followed by Western blotting analysis (A, D) and quantification of GFP-LC3 puncta (B, C, E, F). Asterisk in A indicates a non-specific band. Western blots shown are from one representative experiment of two to three performed for each panel. Quantitative data (mean±SEM) are from 3–5 experiments. *: P<0.05, **: P<0.01.