CPD repair in <i>C. elegans</i> germ cells.

<p>Immunofluorescence on dissected gonads shows that after 160 J/m<sup>2</sup> UVB irradiation, CPD damage (green) is repaired in wild type and <i>csb-1</i> animals, but not in <i>xpa-1</i>, <i>rad-23</i> and <i>rad-23; csb-1</i> animals. Repair was visualized 18 hours after irradiation, as at this time point the majority of CPDs in <i>C. elegans</i> are repaired <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1000941#pgen.1000941-Meyer1" target="_blank">[52]</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1000941#pgen.1000941-Hartman3" target="_blank">[65]</a>, while the proliferation arrest caused by UV irradiation in distal mitotic germ cells still lasts <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1000941#pgen.1000941-Stergiou1" target="_blank">[22]</a>. To visualize germ cell nuclei, DAPI staining (blue) was used. Bar in first image represents 20 µm.</p>