CHC-1 and AP2 are required for phagosome maturation.

(A) Representative images of germ cell corpse staining by LysoSensor Green DND-189 in gla-3(RNAi), chc-1(RNAi) and apb-1(RNAi) animals. Quantification of corpse staining is shown on the right. ≥100 corpses were analyzed for each RNAi treatment. (B–D) Representative DIC and fluorescence images of germ cell corpse labeling by GFP::RAB-5 (B), mCherry::RAB-14 (C), and NUC-1::mCherry (D) in Ctrl(RNAi), apb-1(RNAi), and chc-1(RNAi) animals. (E) Quantification of cell corpse labeling as shown in (B–D). ≥100 corpses were analyzed for each genotype. In (A–D), arrows indicate cell corpses labeled by phagosomal markers; arrowheads indicate unlabeled corpses. Bars, 10 µm.