Figure_3.tif (118.39 kB)

CENP-A^Cnp1 can accumulate on centromeric repeat regions independently of H3K9 methylation.

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posted on 2013-07-31, 02:00 authored by Araceli G. Castillo, Alison L. Pidoux, Sandra Catania, Mickaël Durand-Dubief, Eun Shik Choi, Georgina Hamilton, Karl Ekwall, Robin C. Allshire

(A) ChIP-qPCR-analysis of CENP-A^Cnp1 and CENP-C^Cnp3 levels on centromeric outer repeat sequences (otr dgI) in cells expressing endogenous (Endog. CENP-A^Cnp1) or additional (nmt81-CENP-A^Cnp1 or nmt41-CENP-A^Cnp1) levels of CENP-A^Cnp1. (B) ChIP-qPCR analysis of CENP-A^Cnp1 and H3K9me2 levels on centromeric otr dgI repeats in wild-type and clr4∆ cells expressing endogenous (Endog. CENP-A^Cnp1) or additional levels (nmt41-CENP-A^Cnp1) of CENP-A^Cnp1. Enrichment on dgI was normalized to the signal obtained for the euchromatic gene encoding actin (act1⁺). Error bars indicate S.D. from 3 biological replicates. Mean values marked with different letter (a, b or c) indicate results significantly different from each other, as established by One Way ANOVA and Holm-Sidak test for multiple comparison (P<0.01).