CD45 expression distinguishes LSECs from liver macrophages (MØ).

Multiple populations of CD14⁺⁺ cells are distinguished by CD45 expression (A). LSECs represent a population of CD14⁺⁺ cells found among CD45⁻ cells, whereas MØ express high levels of CD45 as indicated by the oval region. Antigen expression is compared on MØ and LSECs (B). Background staining with isotype-control antibody is shown for the oval MØ region. The corresponding antigen expression found on LSECs is shown by gating on CD14⁺⁺CD45⁻ cells as defined in Fig. 3.