CD133-LV does not transduce normal mouse brain cells, hESC-derived neurons and primary human astrocytes <i>in vitro</i>.
2014-12-26T03:21:50Z (GMT) by
<p><b>A</b>. Injection of CD133-LV expressing mCherry into the mouse basal ganglia did not lead to transduction of normal brain tissue, as opposed to VSVG-LV (BG: basal ganglia, Cx: cortex, CC: corpus callosum, LV: lateral ventricle). <b>Bi,ii</b>. hESC-derived neurons were transduced with either CD133-LV or VSVG-LV expressing mCherry. VSVG-LV led to transduction of MAP2A+ neurons, as opposed to CD133-LV. <b>Ci,ii</b>. Primary human astrocytes were transduced with either CD133-LV or VSVG-LV expressing mCherry (MOI = 10). VSVG-LV led to transduction of GFAP+ astrocytes, as opposed to CD133-LV. (NeuN: neural nuclei, MAP2A: microtubule associated protein 2A, GFAP: glial fibrillary acidic protein). Nuclei were counterstained with DAPI.</p>