BmSpry regulation of ERK activation inhibited BmNPV replication.

(A) Firstly, BmE cells were transfected. The empty vector 1180 was used as a negative control and the BmSpry-oe vector was used as the experimental group with over-expression of BmSpry. At 2 days post-transfection, BmE cells were infected with BmNPV at MOI of 10. Post infection for 24 h, total proteins were extracted for western blotting using anti-phospho-ERK antibody. Total protein levels were measured with anti-GAPDH antibody. (B) BmN4-SID1 cells were used. After RNAi of 5 days, the BmN4-SID1 cells were infected with BmNPV at MOI of 10. Post infection for 24 h, total proteins were extracted for western blotting. A representative of triplicate experiments is shown.