Figure_7.tif (1.26 MB)
Blocking aSyn phosphorylation reduces membrane interactions and accelerates inclusion formation.
figure
posted on 2014-05-08, 02:51 authored by Sandra Tenreiro, Madalena M. Reimão-Pinto, Pedro Antas, José Rino, Donata Wawrzycka, Diana Macedo, Rita Rosado-Ramos, Triana Amen, Meytal Waiss, Filipa Magalhães, Andreia Gomes, Cláudia N. Santos, Daniel Kaganovich, Tiago Fleming Outeiro(A) Time lapse confocal imaging comparing the distribution of WT or S129A aSyn-GFP at the indicated time points after induction of aSyn expression. Nuclei were visualized by co-expressing NLS-TagRFP657. (B) Percentage of WT and S129A aSyn-GFP expressing cells presenting inclusions over time. nwt = 48 cells, nS129A = 55 cells. Inclusions were recognized by measuring GFP intensity.
