Blocking aSyn phosphorylation reduces membrane interactions and accelerates inclusion formation.

<p>(A) Time lapse confocal imaging comparing the distribution of WT or S129A aSyn-GFP at the indicated time points after induction of aSyn expression. Nuclei were visualized by co-expressing NLS-TagRFP657. (B) Percentage of WT and S129A aSyn-GFP expressing cells presenting inclusions over time. n<sub>wt</sub> = 48 cells, n<sub>S129A</sub> = 55 cells. Inclusions were recognized by measuring GFP intensity.</p>