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Biological activity of reporter proteins in physiological buffer or TRIzol lysates.

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posted on 2014-11-18, 04:16 authored by Junichiro Futami, Haruna Fujiyama, Rie Kinoshita, Hidenori Nonomura, Tomoko Honjo, Hiroko Tada, Hirokazu Matsushita, Yoshito Abe, Kazuhiro Kakimi

Hek293 cells expressing either GFP or Luc were directly lysed in physiological Glo Lysis buffer (GLB) or TRIzol, and GFP or Luc activity were measured. Reporter proteins were verified by western blotting.

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    prokaryotic protein mixturesprotein mixturescell protein lysatesDenatured Mammalian Protein Mixtures Exhibit Unusually High Solubility

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