BcLTF1 modulates the transcriptional responses to light.
2014-01-09T03:12:44Z (GMT) by
<p>Wild type B05.10 and the Δ<i>bcltf1</i> mutant were grown on solid CM for 52 h in DD; then, cultures were exposed for 60 min to light (+LP) or kept for additional 60 min in darkness (D). Four biological replicates were performed. RNA samples were labeled and hybridized to microarrays with all predicted genes of <i>B. cinerea</i>. Statistical analysis revealed 2,074 differentially expressed genes. Relative expressions of the genes (i.e. log2-normalized intensities scaled by gene) in the four conditions (16 hybridizations) were clustered, and 11 of the 14 theoretically possible expression profiles were found (for more details, see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004040#s4" target="_blank">Materials and Methods</a>). This figure represents the centroid views of the 11 expression profiles (x = hybridization and y = log2-normalized intensity scaled by gene). The percentages among the 2,074 genes are indicated for each profile. Genes whose expression is only affected by the deletion of <i>bcltf1</i> belong to profiles 1 (748 genes) and 2 (791 genes). Profiles 3 (94 genes) and 4 (5 genes) comprise genes whose expression is affected by light in a similar manner in both genomic backgrounds (WT, Δ<i>bcltf1</i>). Genes (436) belonging to the other profiles are affected by light treatment and the deletion of <i>bcltf1</i>.</p>