Bacterial replication in lungs and spleen of BALB/c mice vaccinated with HMA or BCG and challenged with 10<sup>5</sup> CFU of virulent luminescent <i>M. tuberculosis</i>.

<p>Mean number of bacteria in lungs and spleen of BALB/c mice vaccinated with 5x10<sup>5</sup>CFU of HMA mutant or BCG or unvaccinated mice (naïve), challenged 12 weeks later with 10<sup>5</sup>CFU of virulent <i>M.tb</i> by the intratracheal route and sacrificed 6 or 12 weeks after challenge. Results are reported as mean log<sub>10</sub> relative light units (RLU) ± SD of 5 mice tested individually; ** <i>P</i><0,01; *** <i>P</i><0.001 (Mann-Whitney test). </p>