BMP2 governs osteogenic differentiation of MC3T3-E1 cells.

(A) mRNA Levels of ALP, RUNX2 and SP7 were measured after recombinant BMP2 or/and dorsomorphin (DM) treatment for 12 h. *, BMP2 group versus control group; #, BMP2 + DM group versus BMP2 group. (B) ALP activity was detected as indicated at 24 h pf.. (C) Microscopic examination and (D) quantification of ARS stain were carried out as above to assess the terminal differentiation at day 6 pf.. (Scale bar: 50 µm. Data were shown as mean ± SD. n = 3. ***, P<0.001; ###, P<0.001.)