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Asymmetric pectocin M1 spot test inhibition zones and growth enhancement due to plant-like ferredoxin, but not adrenodoxin

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posted on 2012-03-09, 00:09 authored by Rhys Grinter, Joel Milner, Daniel Walker

a) Asymmetric pectocin M1 inhibition zones against P. atrosepticum LMG2386 due to plant-like ferredoxin containing proteins. Protein concentration for spots were as follows: pectocin M1 1.2 mg ml−1, spinach ferredoxin 20 mg ml−1, adrenodoxin 30 mg ml−1, pectocin M2 10 mg ml−1, pectocin M1 D222A 5.6 mg ml−1 b) Growth enhancement of P. carotovorum subspp. carotovorum LMG 2410 by plant-like ferredoxin containing proteins. Protein concentrations for spots were as follows: spinach ferredoxin 20 mg ml−1, adrenodoxin 30 mg ml−1, Spinach ferredoxin 20 mg ml−1 :pectocin M1 1.2 mg ml−1. Pectocin M1 causes asymmetry in zone of enhancement due to spinach ferredoxin in LMG 2410 (400 µM 2,2′-bipyridine) presumably through competition for a common receptor. Pectocin M1 does not enhance growth of this strain under these conditions.

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